Carrier CPP-PNA: Deca-(D-Arg)8-cPNA4 delivering antisense ssRNA (+ chloroquine)
Sequence: (D-Arg)8_SYS5
| Experiment Id | EXP000309 |
|---|---|
| Paper | Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delive |
| Peptide | Carrier CPP-PNA: Deca-(D-Arg)8-cPNA4 delivering antisense ssRNA (+ chloroquine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 1:1 (RNA:carrier) |
| Formulation Format | |
| Formulation Components | Antisense ssRNA + carrier CPP-PNA Deca-(D-Arg)8-cPNA4 + chloroquine |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | p53R (luciferase-expressing) |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase expression downregulation |
| Output Value | 59% downregulation at 60 nM ssRNA with Deca-(D-Arg)8-cPNA4 + 100 μM chloroquine (reported) |
| Output Units | |
| Output Notes | Without chloroquine: no significant knockdown up to 60 nM; with chloroquine + Deca-(D-Arg)8-cPNA4: significant knockdown (Fig.6). |
| Toxicity Notes | |
| Curation Notes |