Sequence: RXRRXRRXRRXRR
| Experiment Id | EXP002215 |
|---|---|
| Paper | Antisense peptide nucleic acids against ftsZ and efaA genes inhibit growth and biofilm formation of |
| Peptide | (RXR)4 carrier peptide |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP–PNA conjugate |
| Formulation Components | (RXR)4–efaA PNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Enterococcus faecalis ATCC 29212 (bacteria) |
| Animal Model | |
| Administration Route | direct incubation |
| Output Type | gene knockdown and biofilm inhibition |
| Output Value | ~95–99% reduction in efaA expression; significant biofilm reduction |
| Output Units | |
| Output Notes | qRT-PCR and crystal violet biofilm assays |
| Toxicity Notes | No toxicity in MCF7 cells (MTT assay) |
| Curation Notes |