Sequence: rrrqrrkkr
| Experiment Id | EXP002417 |
|---|---|
| Paper | Cell-Penetrating Peptides and Peptide Nucleic Acid-Coupled MRI Contrast Agents: Evaluation of Cellul |
| Peptide | D-Tat57-49 / D-Tatinv |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10 µmol/kg i.v. dose |
| Rna Concentration | 10 µmol/kg anti-dsred PNA-containing conjugate |
| Mixing Ratio | covalent 1:1 peptide:PNA conjugate |
| Formulation Format | Covalent CPP-PNA-MRI/fluorescent contrast-agent conjugate |
| Formulation Components | Gd-DOTA + FITC + anti-dsred PNA + AEEA spacer + D-Tat57-49 |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6 mice |
| Administration Route | intravenous tail-vein injection |
| Output Type | Biodistribution by tissue fluorescence 20 min after injection |
| Output Value | Strong fluorescence in liver and urinary bladder (+++); kidney/spleen moderate (++); lung faint (+); no brain/blood signal |
| Output Units | |
| Output Notes | Preliminary biodistribution only; no in vivo functional RNA delivery, knockdown, reporter expression, phenotypic rescue, or target binding outcome demonstrated. Therefore delivery_success_class=0 despite in vivo testing. |
| Toxicity Notes | No conjugate-related toxicity observed. |
| Curation Notes |