Peptide codes for organ-selective mRNA delivery (2026)
Sequence: RRRRRR
| Experiment Id | EXP002420 |
|---|---|
| Paper | Peptide codes for organ-selective mRNA delivery |
| Peptide | 6R-LNP / Cas9 mRNA + sgPten |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | not separately reported; DSPE-PEG-peptide included by molar ratio in LNP formulation |
| Rna Concentration | 50 ng Luc mRNA per well for in vitro expression assay; 500 ng/well for Cy5 uptake assay when used |
| Mixing Ratio | N/P ratio 5.67 for mRNA encapsulation |
| Formulation Format | Peptide-modified lipid nanoparticle (POST-LNP) |
| Formulation Components | POST-LNP: ionizable lipid (mostly MC3 unless noted), cholesterol, DSPC/helper lipid, DSPE-PEG-peptide, DSPE-PEG, and encapsulated RNA; standard molar ratio 50:38.5:10:6:0.75 unless otherwise specified. |
| Size Nm | 170.90 |
| Zeta Mv | 33.10 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A-498 and U-87 MG αvβ3-integrin-expressing cells |
| Animal Model | |
| Administration Route | |
| Output Type | Vtn-dependent cellular uptake and Luc reporter expression |
| Output Value | functional reporter expression in vitro only |
| Output Units | |
| Output Notes | This row is not an in vivo positive. Uptake was directly measured with fluorescent/Cy5-labeled RNA/LNP assays; delivery_success_class remains 0. |
| Toxicity Notes | POST-LNPs showed minimal organ toxicity by H&E at high Luc mRNA dose compared with LPS; no explicit toxicity issue reported for curated rows. |
| Curation Notes |