Peptide codes for organ-selective mRNA delivery (2026)
Sequence: RRRRRR
| Experiment Id | EXP002422 |
|---|---|
| Paper | Peptide codes for organ-selective mRNA delivery |
| Peptide | 6R-LNP / Cas9 mRNA + sgPten |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | not separately reported; DSPE-PEG-peptide included by molar ratio in LNP formulation |
| Rna Concentration | 0.1 mg/kg Luc mRNA i.v.; organs imaged 3 h after dosing |
| Mixing Ratio | N/P ratio 5.67 for mRNA encapsulation |
| Formulation Format | Peptide-modified lipid nanoparticle (POST-LNP) |
| Formulation Components | POST-LNP: ionizable lipid (mostly MC3 unless noted), cholesterol, DSPC/helper lipid, DSPE-PEG-peptide, DSPE-PEG, and encapsulated RNA; standard molar ratio 50:38.5:10:6:0.75 unless otherwise specified. |
| Size Nm | 170.90 |
| Zeta Mv | 33.10 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6J mice |
| Administration Route | Intravenous injection |
| Output Type | Lung-selective Luc mRNA reporter expression |
| Output Value | in vivo luminescence reporter expression in target organ |
| Output Units | |
| Output Notes | delivery_success_class=1 because functional reporter expression was demonstrated in vivo. uptake_confirmed=1 only because the paper also directly tracked Cy5-labeled mRNA/LNP uptake/distribution for selected POST codes. |
| Toxicity Notes | POST-LNPs showed minimal organ toxicity by H&E at high Luc mRNA dose compared with LPS; no explicit toxicity issue reported for curated rows. |
| Curation Notes |