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EXP002426

Paper

Peptide codes for organ-selective mRNA delivery (2026)

Peptide

6D-LNP / Cas9 mRNA + sgPten

Sequence: DDDDDD

RNA

ASO

All experiment fields

Experiment Id EXP002426
Paper Peptide codes for organ-selective mRNA delivery
Peptide 6D-LNP / Cas9 mRNA + sgPten
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration not separately reported; DSPE-PEG-peptide included by molar ratio in LNP formulation
Rna Concentration 1.5 mg/kg ASO i.v. on days 0, 1 and 2
Mixing Ratio Ionizable lipid/ASO ratio fixed at 15/1 (wt/wt)
Formulation Format Peptide-modified lipid nanoparticle (POST-LNP)
Formulation Components POST-LNP: ionizable lipid (mostly MC3 unless noted), cholesterol, DSPC/helper lipid, DSPE-PEG-peptide, DSPE-PEG, and encapsulated RNA; standard molar ratio 50:38.5:10:6:0.75 unless otherwise specified.
Size Nm 148.40
Zeta Mv -54.60
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model ICR-Tg(CAG-EGFP) mice
Administration Route Intravenous injection
Output Type Spleen-selective EGFP-ASO silencing
Output Value organ-selective reduction of EGFP fluorescence
Output Units
Output Notes Functional in vivo RNA effect (ASO-mediated silencing) was demonstrated. uptake_confirmed left NaN because direct ASO uptake was not separately measured for this row.
Toxicity Notes POST-LNPs showed minimal organ toxicity by H&E at high Luc mRNA dose compared with LPS; no explicit toxicity issue reported for curated rows.
Curation Notes