Peptide codes for organ-selective mRNA delivery (2026)
Sequence: RRR
| Experiment Id | EXP002433 |
|---|---|
| Paper | Peptide codes for organ-selective mRNA delivery |
| Peptide | 3R-LNP / Cas9 mRNA + sgPcsk9 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | not separately reported; DSPE-PEG-peptide included by molar ratio in LNP formulation |
| Rna Concentration | 4 mg/kg total RNA i.v. daily for 3 days; Cas9 mRNA/sgPten 4/1 wt/wt |
| Mixing Ratio | Cas9 mRNA/sgPten ratio 4/1 (wt/wt) |
| Formulation Format | Peptide-modified lipid nanoparticle (POST-LNP) |
| Formulation Components | POST-LNP: ionizable lipid (mostly MC3 unless noted), cholesterol, DSPC/helper lipid, DSPE-PEG-peptide, DSPE-PEG, and encapsulated RNA; standard molar ratio 50:38.5:10:6:0.75 unless otherwise specified. |
| Size Nm | 136.30 |
| Zeta Mv | 0.70 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6J mice |
| Administration Route | Intravenous injection |
| Output Type | Liver-selective Pten editing |
| Output Value | decreased Pten staining/expression in target organ |
| Output Units | |
| Output Notes | delivery_success_class=1 because in vivo target-gene editing/knockdown was demonstrated. uptake_confirmed left NaN because this row is functional editing evidence, not direct uptake imaging. |
| Toxicity Notes | POST-LNPs showed minimal organ toxicity by H&E at high Luc mRNA dose compared with LPS; no explicit toxicity issue reported for curated rows. |
| Curation Notes |