Peptide codes for organ-selective mRNA delivery (2026)
Sequence: RRR
| Experiment Id | EXP002436 |
|---|---|
| Paper | Peptide codes for organ-selective mRNA delivery |
| Peptide | 3R-LNP / Cas9 mRNA + sgPcsk9 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | not separately reported; DSPE-PEG-peptide included by molar ratio in LNP formulation |
| Rna Concentration | 2 mg/kg total RNA i.v. on days 0, 2 and 4; Cas9 mRNA/sgPcsk9 1/1 wt/wt |
| Mixing Ratio | Cas9 mRNA/sgPcsk9 ratio 1/1 (wt/wt) |
| Formulation Format | Peptide-modified lipid nanoparticle (POST-LNP) |
| Formulation Components | POST-LNP: ionizable lipid (mostly MC3 unless noted), cholesterol, DSPC/helper lipid, DSPE-PEG-peptide, DSPE-PEG, and encapsulated RNA; standard molar ratio 50:38.5:10:6:0.75 unless otherwise specified. |
| Size Nm | 111.70 |
| Zeta Mv | 7.50 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6J mice |
| Administration Route | Intravenous injection |
| Output Type | Liver Pcsk9 editing with downstream phenotype |
| Output Value | decreased liver Pcsk9, reduced plasma Pcsk9, and ~50% lower blood cholesterol |
| Output Units | |
| Output Notes | delivery_success_class=1 because gene editing produced a functional in vivo phenotype beyond uptake. |
| Toxicity Notes | POST-LNPs showed minimal organ toxicity by H&E at high Luc mRNA dose compared with LPS; no explicit toxicity issue reported for curated rows. |
| Curation Notes |