Sequence: NH2-CDIFTNSRGKRAGGGGrrrrrrrrr
| Experiment Id | EXP002437 |
|---|---|
| Paper | Systemic peptide mediated delivery of an siRNA targeting α-syn in the CNS ameliorates the neurodegen |
| Peptide | C2-9r |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 pmol siα-syn in cell experiments; FITC-siRNA used for uptake time-course |
| Mixing Ratio | 1:40 siRNA:C2-9r |
| Formulation Format | peptide/siRNA electrostatic complex |
| Formulation Components | C2-9r peptide + siα-syn |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | N2A mouse cholinergic neuronal cells |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | α-syn protein knockdown and uptake imaging |
| Output Value | α-syn reduced ~25% at 24 h and ~80% at 48–72 h by immunocytochemistry; immunoblot showed ~30% of control at 24 h and ~70% reduction at 48 h |
| Output Units | |
| Output Notes | FITC-siRNA detected in neuronal cells beginning at 3 h and plateauing by 48–72 h |
| Toxicity Notes | |
| Curation Notes |