Sequence: NH2-RLTRKRGLKLAGGGGGRRRRRRRRR
| Experiment Id | EXP002438 |
|---|---|
| Paper | Systemic peptide mediated delivery of an siRNA targeting α-syn in the CNS ameliorates the neurodegen |
| Peptide | ApoB11-9R |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 pmol siα-syn in cell experiments; FITC-siRNA used for uptake time-course |
| Mixing Ratio | 1:10 siRNA:ApoB11 |
| Formulation Format | peptide/siRNA electrostatic complex |
| Formulation Components | ApoB11 peptide + siα-syn |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | N2A mouse cholinergic neuronal cells |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | α-syn protein knockdown and uptake imaging |
| Output Value | α-syn reduced ~30% at 24–48 h; nearly complete elimination of immunocytochemistry signal by 72 h; immunoblot showed ~35% of control at 24 h and ~70% reduction at 48 h |
| Output Units | |
| Output Notes | FITC-siRNA detected throughout cytoplasm from 3 h; signal became punctate by 24 h and diminished by 48 h |
| Toxicity Notes | |
| Curation Notes |