Sequence: Ac-grkkrrqrrrppqc-amide
| Experiment Id | EXP002446 |
|---|---|
| Paper | Lung Delivery Studies Using siRNA Conjugated to TAT(48-60) and Penetratin Reveal Peptide Induced Red |
| Peptide | TAT(48-60)-siRNA |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.3–10 µM in L929 cells |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | covalent peptide-siRNA conjugate |
| Formulation Components | TAT(48-60) CPP + p38 MAPK siRNA sequence C |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | L929 mouse fibroblast cell line |
| Animal Model | |
| Administration Route | cell incubation without transfection reagent |
| Output Type | p38 MAP kinase mRNA knockdown |
| Output Value | 36 ± 6% knockdown at 10 µM after 24 h |
| Output Units | |
| Output Notes | In vitro knockdown was modest but significant; free TAT peptide alone did not reduce p38 MAPK mRNA in vitro |
| Toxicity Notes | No significant cellular toxicity at 24 h reported for constructs |
| Curation Notes |