Sequence: HAIYPRH
| Experiment Id | EXP002455 |
|---|---|
| Paper | Systemic delivery of microRNA-21 antisense oligonucleotides to the brain using T7-peptide decorated |
| Peptide | T7 peptide-decorated exosome |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | not reported as peptide concentration; T7 is displayed on exosome surface via T7-Lamp2b expression |
| Rna Concentration | In vitro: FAM-AMO-21 amount fixed at 1 ug/well for uptake assay; loading used 20 ug AMO-21 with 20 ug exosome protein. In vivo: 60 ug AMO-21 electroporated with exosomes per rat before purification; 200 ug exosomes used for fluorescence imaging. |
| Mixing Ratio | AMO-21 loaded into exosomes by electroporation; 20 ug exosome protein mixed with 20 ug AMO-21 in 400 uL PBS for loading assay. |
| Formulation Format | T7 peptide-decorated exosome loaded with AMO-21 by electroporation |
| Formulation Components | T7-Lamp2b-displaying exosomes, AMO-21 anti-miR oligonucleotide |
| Size Nm | 15.00 |
| Zeta Mv | -10.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | C6 rat glioblastoma cells |
| Animal Model | |
| Administration Route | cell culture exposure to AMO-21-loaded T7-exosomes |
| Output Type | cellular uptake and miR-21 inhibition |
| Output Value | T7-exo showed the highest DiI exosome and FAM-AMO-21 signals in C6 cells; flow cytometry showed higher AMO-21 delivery than unmodified and RVG exosomes; T7-exo/AMO-21 reduced miR-21 levels dose-dependently. |
| Output Units | |
| Output Notes | Functional in vitro anti-miR effect was demonstrated, but in vitro activity does not qualify as in vivo delivery success. |
| Toxicity Notes | Exosomes showed lower cytotoxicity than PEI25k in C6 cells and no remarkable hemolysis/aggregation in rat red blood cell compatibility assay. |
| Curation Notes |