(KFF)3K-L bacterial penetration peptide
Sequence: (KFF)3K-L
| Experiment Id | EXP002501 |
|---|---|
| Paper | A Novel Peptide Nucleic Acid against the Cytidine Monophosphate Kinase of S. aureus Inhibits Staphyl |
| Peptide | (KFF)3K-L bacterial penetration peptide |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 40 µM in initial spot assay; 0–40 µM in MEC assay for cmk/deoD; 20 µM used for mechanistic assays |
| Rna Concentration | |
| Mixing Ratio | Peptide-PNA conjugate; no separate mixing ratio |
| Formulation Format | Peptide-conjugated PNA (P-PNA) |
| Formulation Components | (KFF)3K-L peptide covalently conjugated to antisense PNA oligomer |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | S. aureus ATCC 29740; additional S. aureus strains reported in supplemental data |
| Animal Model | |
| Administration Route | |
| Output Type | bacterial growth inhibition / antisense translation inhibition |
| Output Value | intermediate growth inhibitory effect in spot assay |
| Output Units | |
| Output Notes | Spot assay across seven P-PNAs. qRT-PCR showed cmk/deoD mRNA levels were not changed, while lacZ translational fusion assays supported sequence-specific antisense translation inhibition for ASP-cmk1 and ASP-deoD1. |
| Toxicity Notes | No in vitro cytotoxicity assay for mammalian cells in the main P-PNA screen; antibacterial activity assessed against bacteria. |
| Curation Notes |