Sequence: Stearoyl-AGYLLGILLKKLAAAALKKLL-NH2
| Experiment Id | EXP002517 |
|---|---|
| Paper | Introducing a Single-Point Modification to the Cell-Penetrating Peptide PepFect14 Analogue Sequence |
| Peptide | Ile7 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 nM final SCO concentration in cell media |
| Mixing Ratio | CPP/SCO MR7:1 used for Table 2 summary; MR3:1, MR5:1, MR7:1, and MR10:1 were screened |
| Formulation Format | noncovalent CPP/SCO nanoparticle |
| Formulation Components | Ile7 CPP self-assembled with SCO reporter oligonucleotide in ultrapure water after peptide dilution from 10% DMSO/90% ethanol stock. |
| Size Nm | 94.70 |
| Zeta Mv | -16.80 |
| Model Scope | in_vitro |
| Model Type | in vitro splice-correction reporter assay |
| Cell Lines Or Primary Cells | HeLa pLuc-705 and HeLa-eGFP-654 reporter cell lines |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | splice correction / reporter rescue and Cy5-SCO internalization |
| Output Value | uptake or low/insufficient reporter rescue |
| Output Units | |
| Output Notes | Ile7 showed high Cy5-SCO signal but low productive reporter rescue, consistent with altered packing or possible entrapment; labeled as no functional SCO effect. |
| Toxicity Notes | CPP/SCO nanoparticles formed at MR7:1 did not significantly reduce cell viability compared with untreated cells. |
| Curation Notes |