Peptide-Based Nanoparticles to Rapidly and Efficiently “Wrap ’n Roll” siRNA into Cells (2019)
Sequence: LLRLLRWWWRLLRLL
| Experiment Id | EXP002559 |
|---|---|
| Paper | Peptide-Based Nanoparticles to Rapidly and Efficiently “Wrap ’n Roll” siRNA into Cells |
| Peptide | WRAP5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Peptide concentration varied by molar ratio; 400 nM peptide at R=20 with 20 nM siRNA in main cellular assays |
| Rna Concentration | 20 nM siRNA in main reporter screen; dose-response tested 1–20 nM for lead peptides |
| Mixing Ratio | R = 20 peptide:siRNA molar ratio for main experiments |
| Formulation Format | noncovalent peptide-based nanoparticle |
| Formulation Components | WRAP5 mixed with siRNA in 5% glucose aqueous solution; nanoparticle formation by one-step electrostatic/hydrophobic complexation |
| Size Nm | 80.00 |
| Zeta Mv | 28.80 |
| Model Scope | in_vitro |
| Model Type | in vitro reporter/target-gene knockdown |
| Cell Lines Or Primary Cells | U87 glioblastoma reporter cells; additional cell lines tested for WRAP1/WRAP5 where applicable |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | luciferase reporter knockdown |
| Output Value | Dose-dependent firefly luciferase knockdown; >70% knockdown at R=20 for WRAP1/WRAP5/WRAP6; IC50 around 7.5 nM siFLuc for lead peptides. |
| Output Units | |
| Output Notes | Initial WRAP screen using U87 cells stably expressing firefly/NanoLuc reporters; peptide-only and siRNA-only controls did not induce silencing. |
| Toxicity Notes | LDH assay indicated no relevant cytotoxicity for active WRAP/siRNA nanoparticle conditions; RNAiMAX caused ~20% cytotoxicity in the comparison assay. |
| Curation Notes |