Sequence: RRRRRRRR
| Experiment Id | EXP002563 |
|---|---|
| Paper | Arginine-rich, cell penetrating peptide–anti-microRNA complexes decrease glioblastoma migration pote |
| Peptide | R8 / octaarginine |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | R8 amount varied by positive:negative charge ratio; functional transfection used charge ratio 50:1 |
| Rna Concentration | 55 nM anti-miR-21 in functional transfection; 100 nM Cy3-labeled RNA in uptake/association assays |
| Mixing Ratio | +/− charge ratio 50:1 for anti-miR-21/R8 functional experiments; complexation also characterized across ratios |
| Formulation Format | noncovalent peptide/RNA complex |
| Formulation Components | anti-miRNA mixed with acetyl-R8-amide in RNase-free PBS/OPTI-MEM; complexes formed by electrostatic condensation |
| Size Nm | 200.00 |
| Zeta Mv | 8.00 |
| Model Scope | in_vivo |
| Model Type | in vitro anti-miRNA delivery and migration assay |
| Cell Lines Or Primary Cells | U251 human glioblastoma cells |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | anti-miR-21 functional effect, target-gene de-repression, migration inhibition, uptake/endosomal escape |
| Output Value | PDCD4 and SERPINB5 mRNA increased after anti-miR-21/R8; U251 cell migration inhibited by ~25% versus negative-control anti-miRNA/R8; >50% of cells associated with anti-miRNA/R8; anti-miRNA/R8 endosome escape efficiency reported as ~68%. |
| Output Units | |
| Output Notes | Single-stranded anti-miR-21 was the main functional cargo. Cy3-labeled anti-miRNA and Cy3-labeled/control siRNA were used for uptake/escape and physicochemical comparison; siRNA controls were not curated as separate functional peptide–RNA systems. |
| Toxicity Notes | No significant cytotoxicity reported for anti-miR-21/R8 complexes at the tested concentration; changes were attributed to miRNA inhibition rather than cell death. |
| Curation Notes |