Molecular Parameters of siRNA–Cell Penetrating Peptide Nanocomplexes for Efficient Cellular Delivery (2013)
Sequence: Acetyl-RRRRRRRRR-NH2
| Experiment Id | EXP002566 |
|---|---|
| Paper | Molecular Parameters of siRNA–Cell Penetrating Peptide Nanocomplexes for Efficient Cellular Delivery |
| Peptide | R9 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP concentration depended on molar ratio; optimized MR mostly 50, TP10 MR 100 |
| Rna Concentration | 50, 100, and 200 nM siGL3 in functional luciferase assays |
| Mixing Ratio | Optimized molar ratio: MR 50 for all CPPs except TP10 MR 100 |
| Formulation Format | noncovalent CPP–siRNA nanocomplex |
| Formulation Components | R9 mixed noncovalently with siGL3; complexes characterized for size, zeta potential, serum stability, cell association, and luciferase knockdown |
| Size Nm | 9.00 |
| Zeta Mv | 14.80 |
| Model Scope | in_vitro |
| Model Type | in vitro reporter gene silencing / physicochemical CPP-siRNA nanocomplex study |
| Cell Lines Or Primary Cells | SKNO-1 human leukemic cells stably expressing luciferase-IRES-EGFP |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | luciferase reporter knockdown |
| Output Value | ~15% luciferase knockdown at 200 nM siRNA; no substantial cell association detected |
| Output Units | |
| Output Notes | R9 complexed siRNA but did not show significant SKNO-1 cell association or productive siRNA delivery. |
| Toxicity Notes | WST-1 showed no significant metabolic toxicity at 50–200 nM siRNA; LDH membrane toxicity was peptide-dependent and generally increased at higher complex concentrations. |
| Curation Notes |