Molecular Parameters of siRNA–Cell Penetrating Peptide Nanocomplexes for Efficient Cellular Delivery (2013)
Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP002570 |
|---|---|
| Paper | Molecular Parameters of siRNA–Cell Penetrating Peptide Nanocomplexes for Efficient Cellular Delivery |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP concentration depended on molar ratio; optimized MR mostly 50, TP10 MR 100 |
| Rna Concentration | 50, 100, and 200 nM siGL3 in functional luciferase assays |
| Mixing Ratio | Optimized molar ratio: MR 50 for all CPPs except TP10 MR 100 |
| Formulation Format | noncovalent CPP–siRNA nanocomplex |
| Formulation Components | PF14 mixed noncovalently with siGL3; complexes characterized for size, zeta potential, serum stability, cell association, and luciferase knockdown |
| Size Nm | 14.00 |
| Zeta Mv | 46.70 |
| Model Scope | in_vitro |
| Model Type | in vitro reporter gene silencing / physicochemical CPP-siRNA nanocomplex study |
| Cell Lines Or Primary Cells | SKNO-1 human leukemic cells stably expressing luciferase-IRES-EGFP |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | luciferase reporter knockdown |
| Output Value | ~45% luciferase knockdown at 50 nM siRNA and ~70% at 200 nM siRNA |
| Output Units | |
| Output Notes | PF14 showed substantial functional siRNA delivery with serum-resistant complexes and cytosolic distribution by microscopy. |
| Toxicity Notes | WST-1 showed no significant metabolic toxicity at 50–200 nM siRNA; LDH membrane toxicity was peptide-dependent and generally increased at higher complex concentrations. |
| Curation Notes |