Systematic Comparisons of Formulations of Linear Oligolysine Peptides with siRNA and Plasmid DNA (2016)
Sequence: KKKKKKKKKKKKKKKKKKKKKKKK
| Experiment Id | EXP002573 |
|---|---|
| Paper | Systematic Comparisons of Formulations of Linear Oligolysine Peptides with siRNA and Plasmid DNA |
| Peptide | K24 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | not reported as molarity; oligolysine/siRNA complexes prepared by N/P charge ratio |
| Rna Concentration | 2.4 pmol siRNA per well in transfection assay |
| Mixing Ratio | Different N/P ratios screened; siRNA transfection conditions prepared in OptiMEM |
| Formulation Format | noncovalent oligolysine/siRNA complex |
| Formulation Components | siLuc complexed with K24 linear oligolysine peptide |
| Size Nm | 1.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro siRNA delivery/silencing assay |
| Cell Lines Or Primary Cells | Neuro-2A-luciferase stable reporter cells |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | luciferase knockdown |
| Output Value | No significant luciferase knockdown with oligolysine/siLuc complexes; Lipofectamine 2000 control produced ~80% knockdown |
| Output Units | |
| Output Notes | The paper explicitly concludes that K8, K16, K24 and K32 can bind siRNA but do not package it into stable monodisperse complexes, resulting in unsuccessful siRNA uptake/transfection. pDNA-only rows were excluded because the dataset focuses on RNA delivery. |
| Toxicity Notes | No siRNA-specific cytotoxicity concern reported; pDNA K32 complexes showed greater toxicity, but siRNA delivery was unsuccessful. |
| Curation Notes |