Sequence: RRRVVVVVV
| Experiment Id | EXP002575 |
|---|---|
| Paper | Anti-cancer effect of R3V6 peptide-mediated delivery of an anti-microRNA-21 antisense-oligodeoxynucl |
| Peptide | R3V6 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | not reported as molarity; complex prepared by weight ratio |
| Rna Concentration | 100 pmol FAM-labeled antisense-ODN for uptake assay; antisense-ODN amount for functional assay not fully specified in extracted text |
| Mixing Ratio | antisense-ODN:R3V6 weight ratio 1:30 |
| Formulation Format | noncovalent peptide micelle/antisense-ODN complex |
| Formulation Components | R3V6 amphiphilic peptide complexed with anti-miR-21 antisense-ODN |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro uptake and functional anti-miR-21 activity assay |
| Cell Lines Or Primary Cells | C6 rat glioblastoma cells; dual-luciferase-expressing C6 cells for miR-21 activity assay |
| Animal Model | |
| Administration Route | in vitro transfection |
| Output Type | uptake, miR-21 activity inhibition, cell viability/apoptosis |
| Output Value | R3V6 increased FAM-antisense-ODN cellular association more than PEI25k; anti-miR-21/R3V6 increased Renilla/Firefly reporter signal; induced apoptosis and reduced cell viability |
| Output Units | |
| Output Notes | FAM-labeled antisense-ODN/R3V6 uptake was shown by flow cytometry and fluorescence microscopy. Functional anti-miR-21 activity was shown using a miR-21-responsive dual-luciferase assay, and apoptosis was measured by TUNEL. |
| Toxicity Notes | scr-antisense-ODN/R3V6 was less toxic than scr-antisense-ODN/PEI25k; anti-miR-21/R3V6 reduced C6 viability as part of therapeutic effect. |
| Curation Notes |