Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides (2013)
Sequence: VLTTGLPALISWIRRRHRRHC
| Experiment Id | EXP002577 |
|---|---|
| Paper | Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides |
| Peptide | p5RHH |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | p5RHH 10 mM stock diluted 1:200; peptide:siRNA ratio 100:1 |
| Rna Concentration | 50 nM siRNA for GFP knockdown; 25 nM Alexa488-siRNA for uptake assays |
| Mixing Ratio | peptide:siRNA 100:1 |
| Formulation Format | albumin-stabilized peptide/siRNA nanocomplex |
| Formulation Components | p5RHH/siRNA nanoparticles; human serum albumin coating used for stabilized formulation |
| Size Nm | 55.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro uptake, mechanism, and reporter knockdown |
| Cell Lines Or Primary Cells | B16-F10 / B16-GFP melanoma cells |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | uptake, macropinocytosis, endosomal escape, GFP knockdown |
| Output Value | p5RHH/siRNA entered via macropinocytosis, escaped endosomes after acidification, and reduced GFP expression; chloroquine did not further improve knockdown |
| Output Units | |
| Output Notes | Functional siRNA delivery shown by GFP knockdown. Uptake was confirmed by flow cytometry/confocal microscopy with fluorescent siRNA. Endosomal escape was directly supported by bafilomycin inhibition, pH-triggered siRNA release, RBC hemolysis, acridine-orange release, and cytoplasmic siRNA distribution. |
| Toxicity Notes | Scrambled siRNA transfection reported as non-toxic in culture; p5RHH described as low toxicity at tested doses |
| Curation Notes |