Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides (2013)
Sequence: VLTTGLPALISWIKRKRQQRWRRRR
| Experiment Id | EXP002578 |
|---|---|
| Paper | Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides |
| Peptide | p5RWR |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | no |
| Peptide Concentration | not fully specified; used as peptide/siRNA nanoparticles for comparison |
| Rna Concentration | 50 nM siRNA for GFP knockdown comparison |
| Mixing Ratio | not fully specified |
| Formulation Format | peptide/siRNA nanocomplex |
| Formulation Components | p5RWR/siRNA nanoparticles |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vitro mechanistic negative comparator |
| Cell Lines Or Primary Cells | B16-GFP melanoma cells |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | uptake without productive knockdown |
| Output Value | p5RWR showed similar uptake to p5RHH but remained endosomally entrapped and failed to knock down GFP, even with chloroquine-assisted endosomal release |
| Output Units | |
| Output Notes | delivery_success_class remains 0 because the study is in vitro only; in_vitro_functional_effect = 0 because p5RWR did not produce productive GFP silencing. endosomal_escape_evidence = 0 because the paper explicitly shows lack of endosomal disruption/escape for p5RWR nanoparticles. |
| Toxicity Notes | not highlighted as toxic; functionally inactive for siRNA delivery |
| Curation Notes |