Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides (2013)
Sequence: VLTTGLPALISWIRRRHRRHC
| Experiment Id | EXP002580 |
|---|---|
| Paper | Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides |
| Peptide | p5RHH |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | p5RHH stock 10 mM; peptide:siRNA ratio 100:1 |
| Rna Concentration | 25–200 nM siRNA dose range in F8 cells |
| Mixing Ratio | peptide:siRNA 100:1 |
| Formulation Format | albumin-stabilized peptide/siRNA nanocomplex |
| Formulation Components | p5RHH nanoparticles carrying NFKB2 p100/p52 siRNA |
| Size Nm | 55.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro functional NFκB knockdown and viability assay |
| Cell Lines Or Primary Cells | F8 cells, murine model of HTLV-1-induced adult T-cell leukemia/lymphoma |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | protein knockdown and cell viability |
| Output Value | Dose-dependent p100/p52 protein reduction by Western blot; p100/p52 knockdown reduced F8 viability with IC50 around 100 nM |
| Output Units | |
| Output Notes | Functional in vitro delivery demonstrated by NFKB2 protein knockdown and cell viability reduction. Uptake not separately assayed in F8 cells. |
| Toxicity Notes | Scrambled siRNA did not reduce F8 cell viability |
| Curation Notes |