Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides (2013)
Sequence: VLTTGLPALISWIRRRHRRHC
| Experiment Id | EXP002581 |
|---|---|
| Paper | Mechanisms of Nanoparticle-Mediated siRNA Transfection by Melittin-Derived Peptides |
| Peptide | p5RHH |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | p5RHH stock 10 mM; peptide:siRNA ratio 100:1 |
| Rna Concentration | combined siRNA formulation tested across dose range; IC50 ~50 nM for viability effect |
| Mixing Ratio | peptide:siRNA 100:1 |
| Formulation Format | albumin-stabilized peptide/siRNA nanocomplex with two siRNA cargoes |
| Formulation Components | p5RHH nanoparticles simultaneously carrying p65 and p100/p52 siRNAs |
| Size Nm | 55.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro dual-target functional assay |
| Cell Lines Or Primary Cells | F8 cells, murine model of HTLV-1-induced adult T-cell leukemia/lymphoma |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | synergistic cell viability reduction after dual NFκB knockdown |
| Output Value | Dual formulation targeting p65 and p100/p52 reduced F8 cell viability more strongly than either single siRNA, with reported IC50 around 50 nM |
| Output Units | |
| Output Notes | Multiple cargoes were used in the same peptide complex, so this is curated as a separate dual-cargo row. Functional in vitro activity was demonstrated, but no in vivo functional delivery was shown. |
| Toxicity Notes | Scrambled siRNA showed no toxicity/viability reduction |
| Curation Notes |