EGFP-Based Protein Nanoparticles with Cell-Penetrating Peptide for Efficient siRNA Delivery (2015)
Sequence: H6-YGRRARRRRRR-EGFP
| Experiment Id | EXP002291 |
|---|---|
| Paper | EGFP-Based Protein Nanoparticles with Cell-Penetrating Peptide for Efficient siRNA Delivery |
| Peptide | H6-TatEGFP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | protein:siRNA wt/wt ≥10 |
| Formulation Format | protein nanoparticle |
| Formulation Components | H6-TatEGFP protein-only nanoparticle |
| Size Nm | 13.00 |
| Zeta Mv | -0.18 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa tumor cells |
| Animal Model | |
| Administration Route | |
| Output Type | siRNA delivery / cytosolic localization |
| Output Value | Efficient siRNA condensation and intracellular cytosolic/nuclear localization |
| Output Units | |
| Output Notes | Tat-containing nanoparticles deliver siRNA; control H6-EGFP fails |
| Toxicity Notes | Low cytotoxicity up to 400 µg/mL |
| Curation Notes |