New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids (2013)
Sequence: Stearyl-AGYLLG-Orn(δ-link)-INLKALAALAKKIL-NH2
| Experiment Id | EXP000003 |
|---|---|
| Paper | New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids |
| Peptide | NickFect51 (NF51) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 1.0 µM (approx., MR10 with 100 nM siRNA) |
| Rna Concentration | 100 nM (typical; dose-range tested) |
| Mixing Ratio | MR10 (molar ratio peptide:siRNA) |
| Formulation Format | Non-covalent nanoparticles (self-assembled complexes) |
| Formulation Components | Peptide + nucleic acid (mixed in water; used in serum-free or serum-containing media) |
| Size Nm | 94.40 |
| Zeta Mv | -11.80 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | EGFP-CHO |
| Animal Model | |
| Administration Route | |
| Output Type | Gene knockdown (EGFP decrease by FACS; % of untreated) |
| Output Value | |
| Output Units | |
| Output Notes | In vitro RNAi demonstrated; NF51 reported to silence a large fraction of the population (serum reduces performance but still functional). |
| Toxicity Notes | Reported low cytotoxicity at working concentrations (NickFects generally less toxic than Lipofectamine 2000). |
| Curation Notes |