Sequence: RLWMRWYSPRTRAYG (Cys used for PEG-CPP33 conjugation)
| Experiment Id | EXP000011 |
|---|---|
| Paper | Enhanced lysosomal escape of cell penetrating peptide-functionalized metal–organic frameworks for co |
| Peptide | CPP33 |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP-functionalized MOF nanoparticles (ZIF-90) carrying survivin siRNA (Cy3-labeled for imaging) |
| Formulation Components | ZIF-90 + Cy3-survivin siRNA + PEG-CPP33 (surface) |
| Size Nm | 100.00 |
| Zeta Mv | -23.00 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | BALB/c nude mice with subcutaneous A549 xenograft |
| Administration Route | IV tail vein; imaging at 2, 6, 12 h (siRNA dose 2 nmol) |
| Output Type | in vivo biodistribution (fluorescence imaging) |
| Output Value | Stronger tumor accumulation vs Cy3-siRNA@ZIF-90; persists up to 12 h |
| Output Units | |
| Output Notes | Ex vivo organ/tumor imaging at 12 h; liver/kidney signal also observed. |
| Toxicity Notes | |
| Curation Notes |