Sequence: RLWMRWYSPRTRAYG (Cys used for PEG-CPP33 conjugation)
| Experiment Id | EXP000012 |
|---|---|
| Paper | Enhanced lysosomal escape of cell penetrating peptide-functionalized metal–organic frameworks for co |
| Peptide | CPP33 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | siRNA 10–250 nM (MTT); 125 nM (apoptosis assay) |
| Mixing Ratio | |
| Formulation Format | CPP-functionalized MOF nanoparticles (ZIF-90) co-loaded with oridonin + survivin siRNA |
| Formulation Components | ZIF-90 + oridonin (ORI) + survivin siRNA + PEG-CPP33 (surface) |
| Size Nm | 100.00 |
| Zeta Mv | -23.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 (NSCLC) |
| Animal Model | |
| Administration Route | |
| Output Type | apoptosis rate (Annexin V-FITC/PI, 24 h) |
| Output Value | 60.73% apoptosis (PEG-CPP33@NPs; siRNA 125 nM) |
| Output Units | |
| Output Notes | Synergy vs ORI@ZIF-90 (36.45%) and NPs (ORI+siRNA without PEG-CPP33, 51.37%). |
| Toxicity Notes | No significant difference in cytotoxicity of NPs before vs after PEG-CPP33 coupling (24 h). |
| Curation Notes |