Sequence: secondary amphipathic CPP (proprietary sequence)
| Experiment Id | EXP000042 |
|---|---|
| Paper | Pre-administration of PepFect6-microRNA-146a nanocomplexes inhibits inflammatory responses in kerati |
| Peptide | CADY |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP/miRNA non-covalent nanocomplex |
| Formulation Components | CPP (PF6/PF14/CADY) + miR-146a mimic at 2:1 charge ratio |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Human primary keratinocytes (normal human epidermal keratinocytes) |
| Animal Model | |
| Administration Route | |
| Output Type | target gene suppression (RT-qPCR) / uptake (Dy547 microscopy) |
| Output Value | Functional miR-146a effect for PF6/PF14; none for CADY |
| Output Units | |
| Output Notes | Uptake observed but no significant suppression of miR-146a target genes. |
| Toxicity Notes | |
| Curation Notes |