Sequence: stearyl-AGYLLGK(K(K2(tfq4)))INLKALAALAKKIL-NH2
| Experiment Id | EXP000043 |
|---|---|
| Paper | Pre-administration of PepFect6-microRNA-146a nanocomplexes inhibits inflammatory responses in kerati |
| Peptide | PF6 (PepFect6) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP/miRNA non-covalent nanocomplex |
| Formulation Components | CPP (PF6/PF14/CADY) + miR-146a mimic at 2:1 charge ratio |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6 mice, irritant contact dermatitis (PMA-induced ear inflammation) |
| Administration Route | Subcutaneous injection into ear (PF6-miR-146a), then topical PMA |
| Output Type | anti-inflammatory phenotype + target gene suppression |
| Output Value | Reduced ear swelling; reduced pro-inflammatory cytokines/chemokines; Irak1/Card10 down |
| Output Units | |
| Output Notes | PF6-miR-146a pre-administration attenuated inflammation vs PF6-control. |
| Toxicity Notes | |
| Curation Notes |