Endosomal escape and the knockdown efficiency of liposomal-siRNA by the fusogenic peptide shGALA (2011)
Sequence: WEAALAEALAEALAEHLAEALAEALEALAA
| Experiment Id | EXP000108 |
|---|---|
| Paper | Endosomal escape and the knockdown efficiency of liposomal-siRNA by the fusogenic peptide shGALA |
| Peptide | GALA |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 1–4 mol% of total lipid (optimal ~4 mol%) |
| Rna Concentration | 480 nM in vitro; 4 mg siRNA/kg per injection in vivo |
| Mixing Ratio | |
| Formulation Format | PEGylated MEND (liposomal) |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT1080, HeLa-luc |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | |
| Output Units | |
| Output Notes | Enhanced knockdown vs PEG-MEND in vitro |
| Toxicity Notes | |
| Curation Notes |