Endosomal escape and the knockdown efficiency of liposomal-siRNA by the fusogenic peptide shGALA (2011)
Sequence: shortened GALA variant (fusogenic, pH-sensitive)
| Experiment Id | EXP000111 |
|---|---|
| Paper | Endosomal escape and the knockdown efficiency of liposomal-siRNA by the fusogenic peptide shGALA |
| Peptide | shGALA |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 1–4 mol% of total lipid (optimal ~4 mol%) |
| Rna Concentration | 480 nM in vitro; 4 mg siRNA/kg per injection in vivo |
| Mixing Ratio | |
| Formulation Format | PEGylated MEND (liposomal) |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | HT1080 xenograft nude mouse |
| Administration Route | IV |
| Output Type | tumor growth inhibition |
| Output Value | ~58% ACTB knockdown; tumor growth suppression |
| Output Units | |
| Output Notes | |
| Toxicity Notes | No significant hepatic or systemic toxicity |
| Curation Notes |