Sequence: myristic acid-GWTLNSAGYLLGKINLKALAALAKKIL-GGGG-THRPPMWSPVWP
| Experiment Id | EXP000112 |
|---|---|
| Paper | A Myristoylated Cell-Penetrating Peptide Bearing a Transferrin Receptor-Targeting Sequence for Neuro |
| Peptide | myr-TP-Tf |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 20:1 molar ratio (peptide:siRNA) |
| Rna Concentration | 4 pmol siRNA/well (in vitro) |
| Mixing Ratio | 20:1 peptide:siRNA (optimal) |
| Formulation Format | Peptide–siRNA electrostatic complex |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 mg-Luc, b.End3, primary murine neurons/astrocytes |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~60–70% luciferase knockdown; increased hPAP activity |
| Output Units | |
| Output Notes | Efficient uptake, reporter silencing, functional cytosolic siRNA release |
| Toxicity Notes | |
| Curation Notes |