In vitro and in vivo therapeutic siRNA delivery induced by a tryptophan-rich endosomolytic peptide (2014)
Sequence: Ac-RLWRLLWRLWRRLWRLLR-NH2
| Experiment Id | EXP000122 |
|---|---|
| Paper | In vitro and in vivo therapeutic siRNA delivery induced by a tryptophan-rich endosomolytic peptide |
| Peptide | C6M1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 20–60:1 peptide:siRNA molar ratio |
| Rna Concentration | 50 nM siRNA |
| Mixing Ratio | 40–60:1 |
| Formulation Format | Peptide–siRNA electrostatic nanocomplex |
| Formulation Components | ~100–200 nm |
| Size Nm | |
| Zeta Mv | 50.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-K1, A549 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~55–60% GAPDH/Bcl-2 knockdown |
| Output Units | |
| Output Notes | Efficient uptake, pH-dependent membrane disruption, cytosolic siRNA release |
| Toxicity Notes | |
| Curation Notes |