In vitro and in vivo therapeutic siRNA delivery induced by a tryptophan-rich endosomolytic peptide (2014)
Sequence: Ac-RLWRLLWRLWRRLWRLLR-NH2
| Experiment Id | EXP000123 |
|---|---|
| Paper | In vitro and in vivo therapeutic siRNA delivery induced by a tryptophan-rich endosomolytic peptide |
| Peptide | C6M1 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 60:1 peptide:siRNA molar ratio |
| Rna Concentration | 160 µg siRNA/kg per dose |
| Mixing Ratio | 60:1 |
| Formulation Format | Peptide–siRNA electrostatic nanocomplex |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | A549 xenograft nude mouse |
| Administration Route | Intratumoral |
| Output Type | tumor growth inhibition |
| Output Value | ~54% tumor inhibition; Bcl-2 downregulation |
| Output Units | |
| Output Notes | |
| Toxicity Notes | No significant weight loss or systemic toxicity |
| Curation Notes |