Sequence: GALKKLLKKLLKKLLKKLLG
| Experiment Id | EXP000149 |
|---|---|
| Paper | The complex of miRNA2861 and cell-penetrating, dimeric α-helical peptide accelerates the osteogenesi |
| Peptide | LK peptide (ac-LK) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 300–600 nM |
| Rna Concentration | 50 nM |
| Mixing Ratio | miRNA:LK = 1:6 optimal |
| Formulation Format | Electrostatic peptide–miRNA complex |
| Formulation Components | ~674–766 nm |
| Size Nm | |
| Zeta Mv | 14.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Human bone marrow–derived MSCs |
| Animal Model | |
| Administration Route | |
| Output Type | osteogenic differentiation |
| Output Value | |
| Output Units | |
| Output Notes | Enhanced mineralization, Runx2/ALP/OC upregulation via HDAC5 knockdown |
| Toxicity Notes | No cytotoxicity up to 1 μM LK |
| Curation Notes |