TatU1A (photoactivated CLIP-RNAi)
Sequence: YGRKKRRQRRR fused to U1A RBD (aa 2–98)
| Experiment Id | EXP000151 |
|---|---|
| Paper | Cellular siRNA Delivery Mediated by a Cell-Permeant RNA-Binding Protein and Photoinduced RNA Interfe |
| Peptide | TatU1A (photoactivated CLIP-RNAi) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 2 µM TatU1A-Alexa |
| Rna Concentration | 200 nM U1A-fused siRNA/shRNA |
| Mixing Ratio | TatU1A:RNA = 10:1 |
| Formulation Format | Tat-fused RNA-binding protein / siRNA complex (CLIP-RNAi) |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO, dEGFP-CHO, A431 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown (EGFP, EGFR) |
| Output Value | Strong RNAi after photostimulation |
| Output Units | |
| Output Notes | Light-triggered endosomal escape enables cytosolic siRNA release |
| Toxicity Notes | Phototoxicity minimal ≤60 s irradiation |
| Curation Notes |