Peptide dendrimer (APNPs core)
Sequence: (H-Cys-SH)2-Lys-Glu(G2) peptide dendrimer (compound 6)
| Experiment Id | EXP000152 |
|---|---|
| Paper | A tumor-activatable peptide supramolecular nanoplatform for the delivery of dual-gene targeted siRNA |
| Peptide | Peptide dendrimer (APNPs core) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | NPs:siRNA = 15:1 (w/w) |
| Rna Concentration | 1 µg/mL each siRNA |
| Mixing Ratio | hTERT:TRF2 = 1:1 |
| Formulation Format | Peptide dendrimer supramolecular nanoparticle (APNPs–siRNA) |
| Formulation Components | ~121 nm (pH 7.4); ~93 nm (pH 6.5) |
| Size Nm | |
| Zeta Mv | 19.50 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549/PTX, A549, MCF-7/DOX, MCF-7 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / proliferation & invasion inhibition |
| Output Value | |
| Output Units | |
| Output Notes | Simultaneous hTERT (77%) and TRF2 (72%) knockdown; reduced proliferation and invasion |
| Toxicity Notes | Low cytotoxicity in vitro |
| Curation Notes |