Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells (2004)
Sequence: CRQIKIWFQNRRMKWKK
| Experiment Id | EXP000160 |
|---|---|
| Paper | Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells |
| Peptide | Penetratin |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 25 nM siRNA equivalent |
| Rna Concentration | 25 nM |
| Mixing Ratio | 1:1 peptide:siRNA |
| Formulation Format | Disulfide-linked CPP–siRNA conjugate |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | COS-7, CHO-AA8-Luc Tet-Off, C166-GFP, EOMA-GFP |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | |
| Output Units | |
| Output Notes | Efficient luciferase and GFP silencing across multiple cell lines |
| Toxicity Notes | |
| Curation Notes |