Targeting cyclin B1 through peptide-based delivery of siRNA prevents tumour growth (2009)
MPG-8/Chol-MPG-8 (15% Chol coating)
Sequence: Chol-bAFLGWLGAWGTMGWSPKKKRK-Cya
| Experiment Id | EXP000165 |
|---|---|
| Paper | Targeting cyclin B1 through peptide-based delivery of siRNA prevents tumour growth |
| Peptide | MPG-8/Chol-MPG-8 (15% Chol coating) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 0.25–20 nM (dose–response) |
| Mixing Ratio | 20:1 MPG-8:siRNA + 15% Chol-MPG-8 |
| Formulation Format | Stepwise: MPG-8/siRNA (20:1) then coat with Chol-MPG-8 (15%) |
| Formulation Components | 180 ± 45 nm (15% Chol) |
| Size Nm | |
| Zeta Mv | 14.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / G2 arrest |
| Output Value | |
| Output Units | |
| Output Notes | Similar in vitro/ex vivo silencing to MPG-8; cholesterol mainly improves in vivo performance |
| Toxicity Notes | |
| Curation Notes |