Sequence: TAT-TAT-HA-TAT-DRBD (~65 aa DRBD)
| Experiment Id | EXP000198 |
|---|---|
| Paper | Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fu |
| Peptide | PTD-DRBD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10–50 µM PTD-DRBD |
| Rna Concentration | 100–400 nM siRNA |
| Mixing Ratio | |
| Formulation Format | PTD-DRBD/siRNA noncovalent ribonucleoprotein complex |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | H1299, Jurkat T cells, primary T cells, HUVECs, hES cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | |
| Output Units | |
| Output Notes | Rapid, population-wide RNAi without cytotoxicity; superior to lipofection |
| Toxicity Notes | |
| Curation Notes |