Sequence: TAT-TAT-HA-TAT-DRBD (~65 aa DRBD)
| Experiment Id | EXP000199 |
|---|---|
| Paper | Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fu |
| Peptide | PTD-DRBD |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 750 pmol luciferase siRNA |
| Mixing Ratio | |
| Formulation Format | PTD-DRBD/siRNA complex |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | ROSA26 luciferase reporter mouse |
| Administration Route | Intranasal |
| Output Type | in vivo gene silencing |
| Output Value | Strong luciferase knockdown in nasal and tracheal epithelium |
| Output Units | |
| Output Notes | |
| Toxicity Notes | No innate immune activation (IFN-α, TNF-α) |
| Curation Notes |