NF-κB Inhibition Suppresses Experimental Melanoma Lung Metastasis (2020)
Sequence: p5RHH amphipathic peptide (histidine-rich; exact sequence proprietary/previously published)
| Experiment Id | EXP000200 |
|---|---|
| Paper | NF-κB Inhibition Suppresses Experimental Melanoma Lung Metastasis |
| Peptide | p5RHH |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 100:1 peptide:siRNA (molar) |
| Formulation Format | p5RHH–siRNA nanoparticles |
| Formulation Components | ~55 nm |
| Size Nm | |
| Zeta Mv | 33.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | B16-F10; B16-F10-eGFP melanoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | 82% p65 mRNA knockdown; 48% protein knockdown |
| Output Units | |
| Output Notes | Confocal imaging shows cytoplasmic siRNA delivery; free siRNA inactive |
| Toxicity Notes | |
| Curation Notes |