Sequence: GGGRRRRRRRRR (3G9R core); Tat: GRKKRRQRRRPPQ
| Experiment Id | EXP000227 |
|---|---|
| Paper | Self-Assembling Lipid–Peptide Hybrid Nanoparticles of Phospholipid–Nonaarginine Conjugates for Enhan |
| Peptide | Nonaarginine (9R) + Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | N/P = 10 |
| Rna Concentration | 0.1–5 µM |
| Mixing Ratio | N/P = 10 |
| Formulation Format | lipid–peptide hybrid nanoparticle (T-hNP) |
| Formulation Components | PL9R + siRNA + POPC + cholesterol + PEG-PE ± Tat-PEG-PE |
| Size Nm | 170.00 |
| Zeta Mv | 7.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa-GFP; HeLa-Luc; B16F10 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~50% GFP and luciferase knockdown (Tat-dependent kinetics) |
| Output Units | |
| Output Notes | Tat accelerates uptake and early silencing; effect converges over time |
| Toxicity Notes | Low cytotoxicity; improved vs PEI |
| Curation Notes |