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EXP000228

Paper

Self-Assembling Lipid–Peptide Hybrid Nanoparticles of Phospholipid–Nonaarginine Conjugates for Enhanced Delivery of Nucleic Acid Therapeutics (2017)

Peptide

Nonaarginine (9R) + Tat

Sequence: GGGRRRRRRRRR (3G9R core); Tat: GRKKRRQRRRPPQ

RNA

siRNA

All experiment fields

Experiment Id EXP000228
Paper Self-Assembling Lipid–Peptide Hybrid Nanoparticles of Phospholipid–Nonaarginine Conjugates for Enhan
Peptide Nonaarginine (9R) + Tat
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration 7.52 µM siRNA per mouse
Rna Concentration 7.52 µM per mouse
Mixing Ratio N/P = 10
Formulation Format lipid–peptide hybrid nanoparticle (T-hNP)
Formulation Components PL9R + siRNA + lipid envelope + Tat
Size Nm 170.00
Zeta Mv 7.00
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model HeLa-Luc xenograft mouse model
Administration Route intravenous (tail vein)
Output Type tumor gene silencing
Output Value ~55% reduction in tumor bioluminescence
Output Units
Output Notes Sustained silencing up to 48 h; superior to non-Tat hNP
Toxicity Notes No significant toxicity or hemolysis
Curation Notes