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EXP000236

Paper

Suppression of Hepatic Inflammation via Systemic siRNA Delivery by Membrane-Disruptive and Endosomolytic Helical Polypeptide Hybrid Nanoparticles (2016)

Peptide

PPABLG

Sequence: Poly(γ-(4-vinylbenzyl)-L-glutamate) derivative; DP ~206 (helical polypeptide)

RNA

siRNA

All experiment fields

Experiment Id EXP000236
Paper Suppression of Hepatic Inflammation via Systemic siRNA Delivery by Membrane-Disruptive and Endosomol
Peptide PPABLG
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration 50–500 µg siRNA/kg (i.v.)
Rna Concentration 50–500 µg siRNA/kg
Mixing Ratio 20/2/1 (w/w/w)
Formulation Format hybrid polypeptide nanoparticle (HNP)
Formulation Components PPABLG + PAOBLG-MPA + siRNA
Size Nm 100.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model Mouse LPS/D-GalN–induced hepatic inflammation model
Administration Route intravenous
Output Type therapeutic gene silencing and survival
Output Value Systemic TNF-α knockdown; reduced cytokines; 50% survival at 50 µg/kg
Output Units
Output Notes Kupffer-cell–targeted delivery; strong anti-inflammatory rescue
Toxicity Notes Low immune and systemic toxicity
Curation Notes