Targeted Polymeric Micelles for siRNA Treatment of Experimental Cancer by Intravenous Injection (2012)
Sequence: cyclo-[Arg-Gly-Asp-D-Phe-Lys(Cys)]
| Experiment Id | EXP000239 |
|---|---|
| Paper | Targeted Polymeric Micelles for siRNA Treatment of Experimental Cancer by Intravenous Injection |
| Peptide | cRGD (cyclo-Arg-Gly-Asp) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | polymer/siRNA molar ratio ~7.6 |
| Rna Concentration | 200 nM |
| Mixing Ratio | polymer/siRNA molar ratio ~7.6 |
| Formulation Format | targeted polymeric micelle |
| Formulation Components | cRGD-PEG-b-PLL(2IT) + siRNA |
| Size Nm | 45.00 |
| Zeta Mv | -0.50 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa-luc |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | Significant luciferase knockdown (sequence-specific) |
| Output Units | |
| Output Notes | cRGD essential for uptake and RNAi; non-cRGD micelles inactive |
| Toxicity Notes | Well tolerated; no cytotoxicity |
| Curation Notes |