Targeted Polymeric Micelles for siRNA Treatment of Experimental Cancer by Intravenous Injection (2012)
Sequence: cyclo-[Arg-Gly-Asp-D-Phe-Lys(Cys)]
| Experiment Id | EXP000240 |
|---|---|
| Paper | Targeted Polymeric Micelles for siRNA Treatment of Experimental Cancer by Intravenous Injection |
| Peptide | cRGD (cyclo-Arg-Gly-Asp) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 24 µg siRNA per injection (i.v.) |
| Rna Concentration | 24 µg per injection |
| Mixing Ratio | polymer/siRNA molar ratio ~7.6 |
| Formulation Format | targeted polymeric micelle |
| Formulation Components | cRGD-PEG-b-PLL(2IT) + siRNA |
| Size Nm | 45.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | HeLa-luc subcutaneous xenograft mouse model |
| Administration Route | intravenous (tail vein) |
| Output Type | tumor growth inhibition and in vivo gene silencing |
| Output Value | ~50% VEGF mRNA reduction; significant tumor growth suppression |
| Output Units | |
| Output Notes | cRGD required for tumor accumulation and therapeutic RNAi |
| Toxicity Notes | No significant weight loss or systemic toxicity |
| Curation Notes |