Delivery of short interfering RNA using endosomolytic cell-penetrating peptides (2007)
Sequence: LIRLWSHLIHIWFQNRRLKWKKK-amide
| Experiment Id | EXP000245 |
|---|---|
| Paper | Delivery of short interfering RNA using endosomolytic cell-penetrating peptides |
| Peptide | EB1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | peptide:siRNA 10:1–100:1 |
| Rna Concentration | 100 nM |
| Mixing Ratio | 10:1–100:1 |
| Formulation Format | noncovalent CPP–siRNA complexes |
| Formulation Components | CPP + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; HepG2-luc |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | EB1, MPG-NLS, bPrPp show significant luciferase knockdown; penetratin and TP10 inactive |
| Output Units | |
| Output Notes | Biological activity correlates with endosomolytic capacity, not uptake |
| Toxicity Notes | Low toxicity; no LDH leakage |
| Curation Notes |