Scavenger receptor-mediated uptake of cell-penetrating peptide nanocomplexes with oligonucleotides (2012)
Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
splice-correcting oligonucleotide (SCO)
| Experiment Id | EXP000247 |
|---|---|
| Paper | Scavenger receptor-mediated uptake of cell-penetrating peptide nanocomplexes with oligonucleotides |
| Peptide | PepFect14 (PF14) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 1 µM |
| Rna Concentration | 200 nM |
| Mixing Ratio | MR 5 (peptide:SCO) |
| Formulation Format | noncovalent peptide–oligonucleotide nanocomplex |
| Formulation Components | PF14 + SCO |
| Size Nm | 200.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction (luciferase restoration) |
| Output Value | ~120-fold increase in luciferase activity |
| Output Units | |
| Output Notes | Uptake mediated by SCARA3 and SCARA5; activity abolished by poly I, fucoidin, dextran sulfate |
| Toxicity Notes | No significant cytotoxicity |
| Curation Notes |